Common Hydrogen Bond Interactions in Diverse Phosphoryl Transfer Active Sites
Figure 12
Arginine kinase active site mutation.
Selected interactions of the nucleotide in the crystal structure of the transition state analog complex of Horseshoe Crab Arginine Kinase (AK, PDB ID 1M15) [48]. Carbon = green, nitrogen = dark blue, oxygen = red and phosphorus = orange. In AK, Arg280 contacts the O3β oxygen of ADP, an α-oxygen and the Asp324 side chain. In ATP, the O3β oxygen bridges to the γ-phosphate which is mimicked by nitrate in this transition state analog complex. Hydrogen bonds are shown with red dotted lines.