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Common Hydrogen Bond Interactions in Diverse Phosphoryl Transfer Active Sites

Figure 4

Non-catalytic (blue) active sites have a preference positively charged hydrogen bond donors (a–b) whereas in catalytic (red) active sites neutral interactions are favored (c–d).

Mean numbers of hydrogen bonds were measured for non-bridging (a & c) and bridging (b & d) oxygens. The catalytic group is composed of both O—Pγ and Pα—O structure sets. Positive donors include Lys, Arg, and His side chains and neutral donors include Asn, Gln, Trp, Ser Thr,Tyr, Cys side chains, the nucleotide O2′ and O3′ oxygens and all backbone nitrogens (except Pro).

Figure 4

doi: https://doi.org/10.1371/journal.pone.0108310.g004