Runx2-I Isoform Contributes to Fetal Bone Formation Even in the Absence of Specific N-Terminal Amino Acids
Figure 10
Calvarial cells of Runx2-Ineo/neo mice showed defective osteoblast development.
Calvarial cells recovered from wild-type (+/+) and Runx2-Ineo/neo (neo/neo) mice were cultured in osteogenic medium for 14 days and then fixed and stained with Alizarin red to visualize bone nodule formation. Stained culture wells (A) and numbers of bone nodules normalized by culture area (B). Data are means ± SEM (n = 2–6/group); *p<0.05. (C) At the same time that the Alizarin red assay was conducted, cells were lysed, and alkaline phosphatase activity was tested. Data are representative of four independent experiments and are means ± SEM (n = 3–7/group); *p<0.05.