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Runx2-I Isoform Contributes to Fetal Bone Formation Even in the Absence of Specific N-Terminal Amino Acids

Figure 8

Runx2-Ineo/neo mice showed severely delayed bone ossification.

Skeletal tissues of E18.5 fetuses of wild-type (+/+), Runx2-Ineo/+ (neo/+) and Runx2-Ineo/neo (neo/neo) mice were stained with Alcian blue to detect cartilage, and Alizarin red to detect calcified bone. After whole-body images were captured (A), specimens were separated into parts: (B) clavicle and forelimb; (C) skull; (D) hyoid bone; (E) sternum and rib.

Figure 8

doi: https://doi.org/10.1371/journal.pone.0108294.g008