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Runx2-I Isoform Contributes to Fetal Bone Formation Even in the Absence of Specific N-Terminal Amino Acids

Figure 7

Osteoblastic gene expression was diminished in Runx2-Ineo/neo calvarial cells.

Expression of the indicated osteoblastic genes in calvarial cells (A and C) and osteoblast differentiated cells (B and D) from Runx2-I-mutated (A and B; +/+, neo/+, neo/neo, C and D; +/+, TGA/+, TGA/TGA). Quantitative real-time PCR analysis was performed by using the same RNA used for the analyses shown in Figure 3A (neo-possessing mice) and Figure 4D (neo-deleted mice). Data are representative of three independent experiments and are means ± SEM (n = 3/group); *p<0.05, **p<0.01.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0108294.g007