Runx2-I Isoform Contributes to Fetal Bone Formation Even in the Absence of Specific N-Terminal Amino Acids
Figure 6
Osteoblastic gene expression profile during fetal development.
Pregnant C57BL/6 mice were euthanized for examination of fetuses on days 11, 13, 15, 16, 17, and 18 postcoitum. The calvaria of fetuses were enzymatically digested, and collected cells were subjected to the quantitative real-time PCR analyses. Because the fetuses at days 11 and 13 did not yet have firm calvarial structures, we instead used the upper part of head tissue, which might have contained cells other than calvarial cells (e.g., brain and skin cells). Data are means ± SEM (n = 7–9/group).