Runx2-I Isoform Contributes to Fetal Bone Formation Even in the Absence of Specific N-Terminal Amino Acids
Figure 5
Runx2-I was expressed in Runx2-ITGA/TGA mice as N-terminal truncated Runx2.
(A) Anti-Runx2 mAb (clone: 8G5) could recognize Runx2-II, Runx2-I, and their truncated versions. HEK293 cells were transiently transfected with the indicated expression vectors (see Materials and Methods). Forty-eight hours after transfection, nuclear proteins were obtained from the cells and then immunoblotted with anti-Runx2 mAb. (B) Nuclear proteins of calvarial cells freshly prepared from embryos (18.5 dpc) with the genotypes indicated in the figure were immunoblotted with anti-Runx2. The membranes were also blotted with anti-Lamin A/C mAb as an internal control. The data are representative of three independent experiments.