Induction of M2 Polarization in Primary Culture Liver Macrophages from Rats with Acute Pancreatitis
Figure 5
Treg cells reversed the polarization of Liver macrophages longer than IL-4 treatment.
In vitro, macrophages were treated with IL-4 (10 ng/ml) or Treg (macrophages: Treg ≈2∶1) for 4, 8 and 16 hour. The polarization of M1/M2 was assessed using real time PCR and immunofluorescence. A: mRNA level of M1 markers in the macrophages. B: mRNA level of M2 markers in the macrophages. The significance of differing mRNA levels was analyzed while comparing the two groups in the same time point using the indifferent T-test. Error bars indicate the mean ±S.E. *p<0.05.