Promoting Cell Proliferation Using Water Dispersible Germanium Nanowires
Figure 5
Confocal microscopy to study the morphological features of the L929 cells exposed to WDWs.
(A) L929 cells cultured on a 10 µg collagen glass cover slip and stained with phosphorylated FAK (pFAK397), Hoechst and Phalloidin TRICI under a 63X oil immersion lens using a Zeiss LSM 710. (B): L929 cells cultured on a 10 µg collagen glass cover slip exposed to 4µM of Germanium nanowires for 24 hours and stained with phosphorylated FAK (pFAK397), Hoechst and Phalloidin TRICI under a 63X oil immersion lens using a Zeiss LSM 710. (C) Dispersible Germanium Nanowires were added to cultures of MCF-7 cells for 24 Hr. Lysates were prepared and the lysates ran on 12% SDS PAGE gels before probing with antibodies against Actin (Santa Cruz Biological) pERK (Cell signalling technology phosphor-p44/42 (ERK1/2)), RACK1 (BD Transduction Laboratories) and FAK (Santa Cruz Biotechnology FAK(C-20):sc558). Results were presented as a Histogram of relative FAK expression, RACK1 expression and pERK expression.