Mechanism of Inhibition of the Human Sirtuin Enzyme SIRT3 by Nicotinamide: Computational and Experimental Studies
Figure 4
Inhibition of SIRT3 against NAD+ by Isonicotinamide.
Recombinant human SIRT3 was incubated for 0, 10, 20, 30, 60, 120, 180, and 240 min at 37°C in the presence of 100, 375, 750, 1500, 3000 µM NAD+ and 0, 2.5, 10, and 40 mM isoNAM. Reactions were terminated by the addition of developer and samples were analyzed by fluorometry (excitation set at 355 nm and emission at 460 nm). Data are globally nonlinear fitted to mixed inhibition model (Eq. 2) and shown as a double-reciprocal plot of 1/v versus 1/[NAD+].