Mechanism of Inhibition of the Human Sirtuin Enzyme SIRT3 by Nicotinamide: Computational and Experimental Studies
Figure 1
Inhibition of SIRT3/SIRT1 by nicotinamide and its analog.
(A) Nicotinamide/isonicotinamide inhibition assays showing percent change in deacetylation activity as a function of NAM/isoNAM concentration. Data for the SIRT1 enzyme are indicated with blue squares (filled as NAM, and no filled as isoNAM); data for the SIRT3 enzyme are indicated with red circles (filled as NAM, and no filled as isoNAM). (B) The inset table lists the IC50s of the two inhibitors for these enzymes.