Dimethyl Sulfoxide Damages Mitochondrial Integrity and Membrane Potential in Cultured Astrocytes
Figure 7
Effects of DMSO on expression of GLT-1 and GLAST in cultured mouse cortical astrocytes.
(A) Representative micrographs showing immunoreactivity of GLT-1 and GLAST in astrocytes treated with various concentrations of DMSO for 24 h. (B) Mean integrated optical density (MIOD) of immunostaining for GLT-1 and GLAST. (C) Representative western blot bands showing expression levels of GLT-1 and GLAST in astrocytes. (D) Quantitative analysis revealed that GLT-1 and GLAST protein levels were decreased in astrocytes treated with DMSO in a dose-dependent manner. Data are shown as a mean ± SEM of five (for immunostaining) or four (for Western blot) independent experiments performed in triplicate. *P<0.05 and **P<0.01 versus control group.