Fatty Acid Esters of Phloridzin Induce Apoptosis of Human Liver Cancer Cells through Altered Gene Expression
Figure 5
DNA fragmentation of HepG2 cells.
DNA were isolated after incubation of HepG2 cells with 100 µM of fatty acid esters of phloridzin (Pz) in comparison with parent compounds phloridzin, free fatty acids, phloretin (aglycone) or liver cancer drug sorafenib for 24 h. Lane M: molecular-weight marker, lane C: control, lane 1–6: stearic acid ester of Pz, oleic acid ester of Pz, linoleic acid ester of Pz, α-linolenic acid ester of Pz, DHA ester of Pz and EPA ester of Pz, lane 7: sorafenib, lane 8–13: stearic acid, oleic acid, linoleic acid, α-linolenic acid, DHA and EPA, lane 14: phloridzin, and lane 15: phloretin. Shown are representative gel images of three independent experiments.