Crystal Structure of Transglutaminase 2 with GTP Complex and Amino Acid Sequence Evidence of Evolution of GTP Binding Site
Figure 2
Stoichiometry of TG2 in solution.
A. Gel-filtration chromatogram of TG2. A profile obtained from TG2 in buffer containing 20 mM Tris-HCl at pH 8.0 and 150 mM NaCl is shown. Protein size markers are indicated. B. Multi-angle light scattering (MALS) measurement of TG2 in complex with GTP. The x-axis and y-axis indicate the elution volume and molecular mass, respectively. C. Monomeric structure of TG2 in complex with GTP. The active site is shown by a red circle. The GTP binding site is magnified for better visualization.