Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon (Momordica charantia)
Figure 2
Subcellualr distribution of PAP activity in bitter melon cotyledons.
(A) bitter melon of Indian origin was used in the study. (B) Distribution of PAP activity in bitter mellow cotyledons. The cotyledon extract was successively centrifuged at 3,000 g, 18,000 g and 105,000 g. The final pellet (P3-pellet) after ultracentrifugation is generally regarded as the microsomal membranes. Following dialysis and centrifugation of the 105,000 g supernatant, the S3-pellet (contaminated microsomes) and the S3-supernatant (cytosol) were obtained. All three fractions were analyzed for PAP activity. The data presented are the mean of 2 assays for each sample.