Improved Cell-Free RNA and Protein Synthesis System
Figure 5
Optimization of PURE system with the best combination of EF-Ts, Tu, G; EF 4; RF 1,2,3, RRF; GroEL/GroES; BSA and tRNA concentrations as measured by functional Fluc produced.
Fluc activities were measured in relative luminescence unit by luciferase assay and PURE system reaction without supplement was set as control. Error bars are ± standard deviations, with n = 3.