Improved Cell-Free RNA and Protein Synthesis System
Figure 2
Optimization of PURE system as measured by functional Fluc produced by adding macromolecular crowding agents.
(a). Active Fluc produced in the system at different BSA concentrations. (b). The time course (kinetics) of transcription measured by Fluc mRNA yields with the Quant-iT RiboGreen RNA reagent with and without the presence of 15.5 µM BSA. (c). Active Fluc produced in the system at different PEG-6000 concentrations. In (a) and (c) Fluc activities were measured in relative luminescence units by luciferase assay and PURE system reaction without supplement was set as control. Error bars are ± standard deviations, with n = 3.