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Generation and Characterization of the First Immortalized Alpaca Cell Line Suitable for Diagnostic and Immunization Studies

Figure 5

Transfection.

Representative phase contrast and fluorescence images (10x) of ASSCs transfected with a GFP expression plasmid by different methods (Electroporation, LTX, PEI and Fugene HD), along with the efficiency of transfection as measured by cytometry. Each set of transfections was performed three times and standard deviations were negligible.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0105643.g005