The Jak2 Small Molecule Inhibitor, G6, Reduces the Tumorigenic Potential of T98G Glioblastoma Cells In Vitro and In Vivo
Figure 5
G6 induces caspase-dependent apoptosis in T98G cells.
A) Cells were treated with G6 for 48 hours and apoptosis was examined by TUNEL immunofluorescence. Shown is one of three independent results. Scale bar = 50 µm. B) Quantification (n = 3) of the percentage of TUNEL-positive cells. *, p<0.05 vs. DMSO control treated cells as determined by ANOVA. C) Cells were treated for the indicated times with a 10 µM concentration of G6 and Caspase 3/7 activity was measured by a luminescent assay at the indicated times. Each point was measured in triplicate and shown is one of three independent experiments. A significant difference (*, p<0.05) was noted between the 72 hour time points as determined by ANOVA. D) Cells were treated with G6 for 48 hours and full length Caspase-3 expression was measured by Western blot. Shown is one of two representative blots.