The Jak2 Small Molecule Inhibitor, G6, Reduces the Tumorigenic Potential of T98G Glioblastoma Cells In Vitro and In Vivo
Figure 4
Jak2 knockdown reduces the migratory and invasive potential of T98G cells.
A) Jak2 mRNA expression was examined by qRT-PCR. Each sample was measured in triplicate. Shown is one of three representative results. B) Jak2 protein expression was examined by Western blot. C) Quantification of Jak2 protein expression from three independent blots. D) Cells were plated in 24-well inserts and migration was monitored after 24 hours. E) Quantification of migrating cells from four independent experiments. F) Cells were plated in matrigel-coated 24-well inserts and invasion was monitored after 48 hours. G) Quantification of invading cells from four independent experiments. Scale bars represent either 50 µm (D and 10X F) or 100 µm (4X F) *, p<0.05 relative to scrambled shRNA as determined by Student's t test.