The Jak2 Small Molecule Inhibitor, G6, Reduces the Tumorigenic Potential of T98G Glioblastoma Cells In Vitro and In Vivo
Figure 3
G6 reduces migratory and invasive potential of T98G cells.
A) Cells were treated with G6 for 24 hours at the indicated concentrations, washed, and a scratch was made. Cell migration was monitored 24 and 48 hours later. Shown is one of three representative results. B) Cells were treated with G6 for 24 hours at the indicated concentrations, washed, and plated in un-coated 24-well inserts. Cell migration was examined by crystal violet stain after 24 hours. Shown is one of three representative results. C) Cells were treated with G6 for 24 hours at the indicated concentrations and plated in matrigel-coated 24-well inserts. Cell invasion was examined by crystal violet stain after 48 hours. Shown is one of three representative results. D) Quantification (n = 3) of migrating cells. *, p<0.05 vs. DMSO control treated cells as determined by ANOVA. E) Quantification (n = 3) of invading cells. Scale bars represent either 100 µm (A) or 50 µm (B and C). *, p<0.05 vs. DMSO control treated cells as determined by ANOVA.