Endothelial Nitric Oxide Synthase Dimerization Is Regulated by Heat Shock Protein 90 Rather than by Phosphorylation
Figure 5
Inhibition of PP2A increased phosphorylation of Serine 1179 in eNOS dimer, but did not alter the eNOS dimer∶monomer ratio in BAECs.
BAECs were treated with lactacystin or okadaic acid overnight and the samples were run on SDS-PAGE gel at low temperature (4°C) followed by western blot to determine eNOS phosphorylation and dimer∶monomer ratio as described in the Materials and Methods.