Endothelial Nitric Oxide Synthase Dimerization Is Regulated by Heat Shock Protein 90 Rather than by Phosphorylation
Figure 4
The PI3K signal pathway is the major pathway causing eNOS Serine 1179 phosphorylation induced by VEGF.
After BAECs were treated with MG132 and Ro318220 or LY294002 overnight followed by VEGF challenge, the cell lysates were harvested for the western blotting. The eNOS Ser1179 and Thr497 phosphorylation density were determined by AlphaImager. The results are representative of three independent experiments. (*P<0.05 by one way ANOVA for monomers relative to untreated control and #P<0.05 by one way ANOVA for dimers relative to untreated control).