Endothelial Nitric Oxide Synthase Dimerization Is Regulated by Heat Shock Protein 90 Rather than by Phosphorylation
Figure 2
Inhibition of proteasome 26S resulted in an eNOS monomer accumulation in BAECs.
BAECs were incubated with indicated concentration of lactacystin overnight. The protein was harvested with RIPA buffer and run on SDS-PAGE gel at low temperature (4°C) and the eNOS monomer and dimer content was assessed by western blot and quantified using densitometry. (A) Lactacystin caused a dose-dependent accumulation of eNOS monomer but not dimer; (B) The eNOS monomer and dimer in BAECs were normalized with the controls (0 h) and represented as mean ± SE from four independent experiments. * P<0.05 comparison with control.