Immunoprecipitation of Amyloid Fibrils by the Use of an Antibody that Recognizes a Generic Epitope Common to Amyloid Fibrils
Figure 7
Use of the IP protocol to detect Aβ amyloid fibrils in cerebrospinal fluid (CSF).
(A) Different amounts of Aβ1–40 amyloid fibrils were added to human CSF and the samples were processed as described in the schematic of Figure 5A. As observed by western blot using the Aβ antibody 6E10, picograms of Aβ fibrils were detected in the eluted fraction of the immunoprecipitated sample. (B) The same experiment described in the panel A was conducted with CSF from patients diagnosed with Alzheimer’s disease and the respective age-matched control. A representative example of one of three CSF samples tested is shown. As positive control, we spiked 750 pg of Aβ1–40 amyloid fibrils into human CSF from healthy controls. The absence of detection of soluble Aβ in the CSF is due to its digestion by PK.