Is Thymidine Glycol Containing DNA a Substrate of E. coli DNA Mismatch Repair System?
Figure 5
The MutS interaction with DNA duplexes containing FRET pair.
A, Models of MutS localization on DNA relative to fluorophores Alexa-594 (red) and Alexa-488 (green). The central nucleoside pair is indicated under the cartoons. MutS subunit A which interacts with the mismatch specifically is shown in blue-green; subunit B which forms only non-specific contacts with DNA is shown in yellow-red. B and C, Change in fluorescence anisotropy (Δr) upon maximal binding extent of MutS (total concentration per monomer 125 nM) to DNA (20 nM) containing various central nucleotide pairs: B, for Alexa-488; C, for Alexa-594. Error bars are standard deviations of the mean.