In Vitro Anti-Influenza Virus Activities of a New Lignan Glycoside from the Latex of Calotropis gigantea
Figure 7
Inhibition of influenza virus-induced NF-κB activation in A549 cells by compound 1.
Compound 1 inhibited the influenza virus-induced NF- κB activation (A–J) but did not affect virus-induced Raf/MEK/ERK pathway activation (K). (A) and (K): A549 cells were infected with A/PR/8/34 (H1N1) (MOI = 0.1) in the absence or presence of different concentrations of compound 1 or the specific NF-κB inhibitor Bay11-7085 (10 µM) alone. A549 cell lysates were subjected to Western blot with specific antibodies against phospho-NF-κB p65 (Ser536), total NF-κB p65, phosphorylated ERK1/2 and ERK1/2. Equal protein load was verified using pan-antisera to GADPH. (B–J): A549 cells were infected with A/PR/8/34 (H1N1) (MOI = 1) and stained for against phospho-NF-κB p65 (Ser536) at 10 h of post-infection (green). Cell nuclei were stained with DAPI (blue).