ProSAAS-Derived Peptides Are Differentially Processed and Sorted in Mouse Brain and AtT-20 Cells
Figure 6
Effect of mutation of the two major furin cleavage sites on the localization of SAAS and PEN in AtT-20 cells.
Wild type rat proSAAS was transiently expressed in AtT20 cells and peptides examined by immunofluorescence as described in Materials and Methods using antiserum to PEN (Cy3) and SAAS (Alexa 488 directly labeled). A: Wild type rat proSAAS. B: Rat proSAAS with the two major furin sites (RxRR) mutated to KxRR, which eliminates the furin consensus site. C. Quantification of colocalization as measured by Pearson's Coefficient. At least 30 cells were averaged per condition. Error bars represent standard error of the mean. *, p<0.05 using Student's t-test.