ProSAAS-Derived Peptides Are Differentially Processed and Sorted in Mouse Brain and AtT-20 Cells
Figure 5
Localization of proSAAS-derived peptides in AtT-20 cells.
Immunofluorescence was performed as described in Materials and Methods. A: Big LEN (Cy2) and PEN-LEN (Cy3). B: Big LEN (Alexa 488 directly labeled) and PEN (cy3). C: Big LEN (Alexa 488 directly labeled) and little LEN (Cy3). D: SAAS (Alexa 488 directly labeled) and big LEN (Cy3). E: SAAS (Alexa 488 directly labeled, left panel) and PEN (Cy3, middle panel). The right panel is a merge of the two images. F. SAAS (Alexa 488 directly labeled, left panel) and little LEN (Cy3, middle panel). Right panel is a merge. Scale bars in panels A, B, C, and D indicate 10 µm. Scale bars in panels E and F represent 1 µm.