Differential Activity of Striga hermonthica Seed Germination Stimulants and Gigaspora rosea Hyphal Branching Factors in Rice and Their Contribution to Underground Communication
Figure 5
Characterization of rice mutant d10 exudate.
Germination assay with S. hermonthica seeds on exudate fractions of full nutrition (black bars) and phosphate starvation (white bars) treated plants (A). Water and GR24 (0.33, 3.3 and 33 µM) were used as controls. Peak areas of ent-2'-epi-5-deoxystrigol, (−)−orobanchol and putative methoxy-5-deoxystrigol isomers 2 and 3 (B) and putative methoxy-5-deoxystrigol isomer 1 (C) obtained with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis using multiple reaction monitoring (MRM) of root exudates of d10-2 (black bars) and WT (white bars) under phosphate starvation treatment.