In Vivo Imaging of Hedgehog Pathway Activation with a Nuclear Fluorescent Reporter
Figure 2
Tg(8xGliBS:mCherry-NLS-Odc1) zebrafish exhibit enhanced reporter turnover.
(A–B) Brightfield and fluorescence images of 10-somite (∼14 hpf) Tg(8xGliBS:mCherry-NLS) and Tg(8xGliBS:mCherry-NLS-Odc1) embryos. (C–F) The transgenic lines at 30 hpf after the addition of cyclopamine or vehicle control at the 10-somite stage. The embryos were concurrently treated with phenylthiourea (0.003%, w/v) to block pigmentation. Brightness-enhanced fluorescence images are also shown to more clearly depict mCherry-positive cells in cyclopamine-treated embryos. The zebrafish embryos in (A) and (B) are the same animals shown in (D) and (F), respectively. Embryo orientations: A–B, dorsal view and anterior left; C–F, lateral view and anterior left. Scale bars: A–B, 100 µm; C–F, 200 µm.