Arsenic Trioxide Reactivates Proteasome-Dependent Degradation of Mutant p53 Protein in Cancer Cells in Part via Enhanced Expression of Pirh2 E3 Ligase
Figure 2
Arsenic trioxide induces expression of Pirh2 E3 ligase.
(A) The level of Pirh2 transcript is increased by ATO. RT-PCR analysis was performed with total RNA isolated from MIA PaCa-2 and HaCaT cells untreated or treated with 7.5 µM ATO for 2–6 h. Actin mRNA was amplified as a loading control. (B) Western blots were prepared with extracts from MIA PaCa-2 and HaCaT cells untreated or treated as in (A), and then probed with antibodies against Pirh2 and actin, respectively.