Mechanochemical Phosphorylation and Solubilisation of β-D-Glucan from Yeast Saccharomyces cerevisiae and Its Biological Activities
Figure 5
Effects of native β-D-glucan and three β-D-glucan phosphates, GP-2, GP-4, and GP-5, on cell proliferation (A) and neutral red uptake (B) of RAW264.7 cells. RAW264.7 cells were treated with NG or GP (50, 100, and 500 µg/mL) or LPS (10, 100, and 1000 ng/mL) in different concentrations as described in the Materials and Methods.
After incubation, the viability of RAW264.7 cells was measured by an MTT assay, and the A570 value was recorded, whereas the amount of neutral red uptake was detected by the A540 value. The data represent the means ± SD. *p<0.05, **p<0.01 compared with control. Each point represents the average of three independent experiments.