Mung Bean Nuclease Treatment Increases Capture Specificity of Microdroplet-PCR Based Targeted DNA Enrichment
Figure 2
Mung bean nuclease treatment significantly increases on-target efficiency for DNA enriched through RainDance microdroplet-PCR.
Aliquots of RainDance enriched DNA for the same sample were either treated or not treated with mung bean nuclease, processed into TruSeq libraries, and sequenced on MiSeq as illustrated in Figure 1A. Plotted is the mean value of on-target efficiency of 3 samples that went through parallel treatments (also see Table 2). Nuclease treatment leads to significantly higher on-target efficiency (*p = 0.018, one-tail paired t test; error bar, SEM).