Interferon Regulatory Factor-5 Deficiency Ameliorates Disease Severity in the MRL/lpr Mouse Model of Lupus in the Absence of a Mutation in DOCK2
Figure 1
The experimental mice used in this study did not have the DOCK2 mutation.
PCR to detect the DOCK2 mutation was performed on tail DNA of all mice used in this study. Experimental mice are indicated by number. DNA from a mouse with the DOCK2 mutation (Mu) was used as a positive control and gave a PCR product for the DOCK2 mutation (305 bp). DNA from a mouse without the DOCK2 mutation (WT) was used as a negative control. CD19 PCR (477 bp) was used as an internal control to verify the adequacy of DNA preparation in each sample.