Phytophthora ×stagnum nothosp. nov., a New Hybrid from Irrigation Reservoirs at Ornamental Plant Nurseries in Virginia
Figure 2
Internal transcribed spacer (ITS) sequence alignment of Phytophthora ×stagnum, P. mississippiae and P. taxon PgChlamydo.
Position numbers are given based on the alignment. Yellow indicates sequences belong to P. taxon PgChlamydo authentic isolates. Blue indicates sequences belong to P. mississippiae type isolate 57J3. aPosition 15 is in the poly(A) region of ITS 1, which may contain sequencing errors. Thus, it is excluded from the analysis of hybridization. However, the indel of position 15 among three types of P. ×stagnum clones explains continual polymorphism and unreadable sequences of the ITS 1 regions amplified with the forward primer ITS6F in the initial sequencing before cloning. bType 1 and 2 occurred 21 and 10 times among 31 clones producing 816-bp sequences.