Myotonia Congenita-Associated Mutations in Chloride Channel-1 Affect Zebrafish Body Wave Swimming Kinematics
Figure 3
Maps of α-actin:hCLCN1-EGFP and α-actin:hCLCN1-IRES-EGFP constructs used in this study.
(A–C) Three constructs expressing hCLCN1, hCLCN1I553F/H555N, and hCLCN1L844F –green fluorescent protein (EGFP) fusions under the control of the α-actin promoter were used to produce stable transgenic zebrafish lines. EGFP was fused to the C-terminus of the flag-tagged hCLCN1. The Tol2 vector sequences are shown as thick black lines. (D-E) Three constructs expressing hCLCN1, hCLCN1I553F/H555N, hCLCN1L844F under the control of the α-actin promoter were used to produce stable transgenic zebrafish lines. An internal ribosome entry site (IRES) element is inserted in front of EGFP to allow simultaneous expression of hCLCN1 and EGFP protein separately but from the same RNA transcript. The Tol2 vector sequence is shown as thick black lines.