Multi-Agent Chemotherapy Overcomes Glucocorticoid Resistance Conferred by a BIM Deletion Polymorphism in Pediatric Acute Lymphoblastic Leukemia
Figure 4
Methotrexate, vincristine, and L-asparaginase activate apoptosis in a BIM-independent manner, and overcome BIM deletion-mediated GC resistance.
CCRF-CEM clones were treated with dexamethasone (DEX) (0.1 µM) with or without (A) methotrexate (MTX) (1 µM), (B) vincristine (VCR) (2 ng/ml), or (C) L-asparaginase (ASP) (0.5 IU/ml) for 48 h. Following incubation, cell lysates were obtained and analyzed for cleaved PARP and caspase 3, as well as BIM induction. β-actin was used as a loading control.