Macrophage-Activating Lipopeptide-2 Requires Mal and PI3K for Efficient Induction of Heme Oxygenase-1
Figure 2
MALP-2-stimulation induces c-Src phosphorylation and HO-1 expression.
A. THP-1 cells were stimulated with MALP-2 (5 ng/ml) for the indicated time points and lysed. The lysates were analyzed by Western blotting with a phosphorylated (p)-c-Src antibody (upper panel) or total c-Src antibody (lower panel). The results of a representative experiment are shown, and densitometric quantification of relative protein levels of p-c-Src from three experiments are presented under the panels. B. Cells were preincubated with PP1 for 1 h prior to stimulation with MALP-2. Cell lysates were prepared and HO-1 proteins were detected by Western blotting. C. Cells were transfected with c-Src specific siRNA or control (con) siRNA prior to 5.0 ng/ml MALP-2 treatment. Total c-Src and HO-1 protein expression levels were detected by Western blotting. Results shown are representative of at least three separate experiments. **, P<0.01 for significant difference between compared groups.