Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms
Figure 7
IFN-γ and AECsup induce an oxidative burst following infection with BCG.
BMM were treated with IFN-γ (20 ng/ml) or AECsup 24 h prior to infection and then infected with GFP-BCG. a) ROS production was monitored in the cultures for 16 h by measuring luminescence with luminol as substrate. b) The effect of incubating cells with superoxide dismutase (SOD) and catalase on intracellular killing of BCG is shown. Values are expressed as means ± SD from 5 wells. Differences between treatments with or without SOD/catalase were analyzed using a paired t-test. * significant effect of SOD/catalase treatment, P<0.05.