Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms
Figure 6
AECsup induces IL-1β expression and secretion without an effect of intracellular killing of BCG.
BMM and PuM were infected with GFP-BCG. After infection, cells were treated with either IFN-γ or AECsup or left untreated. a) Total RNA was extracted from both cell-types after 4 and 24 h of treatment and the mean-fold accumulation of IL-1β transcripts shown. Data shows the average of 3 independent experiments. b) Cell culture supernatants were collected 24 h after infection and IL-1β measured with ELISA. Data shows the average of 6 independent experiments. c) The effect of incubating cells with IL-1β (1000 pg/ml) on intracellular growth of BCG. Data representative of 3 independent experiments d) The effect of AECsup on intracellular growth of BCG in BMM from caspase1-/- mice. Bacterial growth was evaluated by determining RLU. Values are expressed as means ± SD from 5 wells. The data is representative of 2 independent experiments. The differences between groups of BMM were analyzed using a one-way ANOVA followed by Bonferroni‘s Multiple Comparison Test. * significantly different from medium control, P<0.05.