Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms
Figure 5
AECsup has opposite effects to IFN-γ on iNOS, and Arg-1 expression in BMM and PuM and mediates killing through an iNOS independent mechanism.
BMM and PuM were infected with GFP-BCG. After infection, cells were treated with either IFN-γ or AECsup or 1 mM of NG-monomethyl-L-arginine (NMMLA) together with either IFN-γ or AECsup or left untreated. Total RNA was extracted from both cell-types after 4 and 24 h of treatment and the mean-fold accumulation of a) iNOS, b) Arg-1 ± SD from 3-4 experiments. In c) the effect of the iNOS inhibitor NMMLA on intracellular growth of BCG is shown. Bacterial growth was evaluated by determining RLU. Data are expressed as mean ± SD. The differences between groups of BMM and PuM were analyzed using non-parametric, one-way ANOVA (Kruskal-Wallis) with Dunn‘s post-test. * significantly different from medium control, P<0.05