Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms
Figure 4
Impaired intracellular control of BCG growth by PuM upon IFN-γ treatment is regulated by SOCS1.
PuM from IFN-γ-/- SOCS1-/- mice were infected with GFP-BCG. After infection, cells were left untreated or treated with IFN-γ, AECsup and IFN-γ + AECsup for 48 h. Bacterial growth was evaluated by determining RLU. Data are shown as % reduction of phagocytosed bacteria evaluated as RLU. Values are means ± SD of the mean of a representative experiment from 2 independent experiments with 4 replicates. The differences between groups of IFN-γ-/- SOCS1-/- PuM were analyzed using a one-way ANOVA followed by Bonferroni‘s Multiple Comparison Test. * significantly different from medium control, P<0.05.