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Macrophage Control of Phagocytosed Mycobacteria Is Increased by Factors Secreted by Alveolar Epithelial Cells through Nitric Oxide Independent Mechanisms

Figure 2

PuM are specifically unresponsive to IFN-γ in the control of intracellular bacteria.

PuM, AM and PEM were infected with GFP-BCG. After infection, cells were treated with either IFN-γ or AECsup for 48 h or left untreated. Bacterial growth was evaluated by determining RLU. Data are shown as % reduction of phagocytosed bacteria evaluated as RLU. Values are means ± SD of the mean of 2 independent experiments with 4 replicates each. The differences between groups of PuM, AM and PEM were analyzed using a one-way ANOVA followed by Bonferroni‘s Multiple Comparison Test, * significantly different from medium control, P<0.05.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0103411.g002