Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection
Figure 11
Relative quantification of RNAs in C. papaya leaf samples.
mRNAs were isolated from leaves, and the accumulation of a group of RNAs associated with stress response pathways was assessed by RT-PCR using a SYBR Green (Life Technologies) with a set of two PCR primers that flank the target region to analyse target genes (Life Technologies). Comparison of the four samples: i) No PMeV load, ii) Very low PMeV load, iii) High PMeV load and iv) Very high PMeV load. The mRNA levels were normalized to cyclophilin (CYP) mRNA. The normalised mRNA level of No PMeV load (control) was set as 1.0, and the other PMeV loads were calculated in relation to the control.