Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection
Figure 10
Relative quantification of miRNAs related to stress response pathways in C. papaya leaf samples.
MiRNAs were isolated from leaves, and the accumulation of a group of miRNAs associated with stress response pathways was assessed by RT-PCR using a TaqMan Small RNA Assay kit (Life Technologies). Comparison of the four samples: i) No PMeV load, ii) Very low PMeV load, iii) High PMeV load and iv) Very high PMeV load. The miRNA levels were normalized to cyclophilin (CYP) mRNA. The normalised miRNA level of No PMeV load (control) was set as 1.0, and the other PMeV loads were calculated in relation to the control.