Carica papaya MicroRNAs Are Responsive to Papaya meleira virus Infection
Figure 9
Relative quantification of miRNAs related to the UPS system in C. papaya leaf samples.
MiRNAs were isolated from leaves, and the accumulation of a group of miRNAs associated with proteasome-ubiquitin degradation was assessed by RT-PCR using a TaqMan Small RNA Assay kit (Life Technologies). Comparison of the four samples: i) No PMeV load, ii) Very low PMeV load, iii) High PMeV load and iv) Very high PMeV load. The miRNA levels were normalized to cyclophilin (CYP) mRNA. The normalised miRNA level of No PMeV load (control) was set as 1.0, and the other PMeV loads were calculated in relation to the control.