Regulation of the Innate Immune Response by Fibronectin: Synergism between the III-1 and EDA Domains
Figure 7
FnEDA and FnIII-1c domains work synergistically to regulate IL-8 expression.
Monolayers of human dermal fibroblasts were serum-starved overnight and then incubated with FnIII-1c and FnEDA alone or in combination. After 4 h (A, B, C) or 24 h (D), the amount of IL-8 protein in the conditioned medium was determined by ELISA. Dashed lines and arrows in (C) indicate the approximate Km of FnIII-1c on IL-8 production. Specific amounts of each module are provided in the Figure. Error bars indicate mean ± S.D. of three experiments performed in triplicate.