Regulation of the Innate Immune Response by Fibronectin: Synergism between the III-1 and EDA Domains
Figure 1
FnIII-1c activates TLR4-dependent p38 MAPK/MK-2 signaling in dermal fibroblasts.
Fibroblasts were serum-starved overnight and treated with the indicated amounts of Fn Type III domains, FnIII-1c, FnIII-10n or FnIII-13 for 1 h. Cell layers were lysed and proteins were electrophoresed and immunoblotted with antibodies to phospho-p38 (A, C) and phospho-MK-2 (B, D). Cells were pretreated with increasing concentrations of antibodies to human TLR4 or 20 µg/ml control antibody, goat IgG (GIgG) for 30 min prior to the addition of 20 µM FnIII-1c for 1 h. Cells were lysed and immunoblotted for phospho-p38 and phospho-MK-2 (E). Immunoblotting for total p38, MK-2 or FAK served as loading controls.