Catestatin Increases the Expression of Anti-Apoptotic and Pro-Angiogenetic Factors in the Post-Ischemic Hypertrophied Heart of SHR
Figure 5
HIF-α expression after 30-min ischemia and 120-min reperfusion.
Representative RT-PCR (panel A) and Western Blots (panel B) and relative densitometry for hypoxia-inducible factor-1α (HIF-1α). Individual values were compared to loading control and the mean value of the Sham group was considered as the reference for all groups, including Sham. Immunohistochemical localization of HIF-1α in the ventricular sections of SHR_Sham (D), SHR_I/R (E), SHR_CST-Post (F) rat. In E the inset shows a detail of HIF-1α labeled myocardiocytes. (C) Negative control. Blue arrows (cardiomyocytes), red arrows (vascular endothelium). *p<0.01. ANOVA followed by Bonferroni’s Multiple comparison test, (n = 6 for each group).